Composite

Part:BBa_K2911002:Design

Designed by: Ryan Lambert   Group: iGEM19_USAFA   (2019-10-14)


Insert consisting of prmA promoter and mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 148
    Illegal AgeI site found at 735
    Illegal AgeI site found at 847
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Insert needed to be expressible in both E. Coli and Rhodococcus RHA1.


Source

Part based off of typical iGEM BioBrick with prmA promoter

References